Super-Resolution Optical Fluctuation Imaging Simulator

Supervisor(s): Prof. Yonina Eldar, Oren Solomon
Abstract: For 120 years, people believed that Abbe’s diffraction law in far field optics, which sets a minimal separation distance for two adjacent objects in an image, cannot be circumvented. 2014 stood out as a celebrated year for super-resolution microscopy, in which three Nobel Prize winners proved us wrong and were acknowledged for their contribution to super-resolution optical fluorescence microscopy. Today, super-resolution imaging techniques such as STORM and PALM enable biologists and other researchers see beyond the diffraction limit and observe intra-cellular entities and dynamics within living cells. Due to several limitations within these techniques, a new technique termed SOFI emerged, which utilizes not only spatial information, but also temporal information in order to construct super-resolution images of cells.
Description: PDF